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FYB anticorps (AA 755-768)

Cet anticorps Lapin Polyclonal détecte spécifiquement FYB dans WB et IP. Il présente une réactivité envers Humain, Souris, Rat, Chien, Cheval, Boeuf (Vache), Porc, Roussette (Chauve-souris) et Singe.
N° du produit ABIN211342

Aperçu rapide pour FYB anticorps (AA 755-768) (ABIN211342)

Antigène

Voir toutes FYB Anticorps
FYB (FYN-Binding Protein (FYB))

Reactivité

  • 46
  • 7
  • 6
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Humain, Souris, Rat, Chien, Cheval, Boeuf (Vache), Porc, Roussette (Chauve-souris), Singe

Hôte

  • 38
  • 7
  • 1
Lapin

Clonalité

  • 39
  • 7
Polyclonal

Conjugué

  • 27
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp FYB est non-conjugé

Application

  • 30
  • 21
  • 13
  • 13
  • 8
  • 7
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB), Immunoprecipitation (IP)
  • Épitope

    • 15
    • 8
    • 8
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 755-768

    Specificité

    Recognizes ADAP/SLAP-130/Fyb, Mr ~130kD. An unknown protein was also detected, Mr ~40kD that is also inhibited by the immunizing peptide. Species sequence homology: Human and rat.

    Homologie

    Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Horse, Pig (100%) Platypus (93%).

    Purification

    Protein A purified

    Immunogène

    Synthetic peptide corresponding to amino acids 755-768 (DNDGEIYDDIADGC) of mouse Fyn binding protein (ADAP/SLAP-130/Fyb) (KLH). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Horse, Pig (100%), Platypus (93%).

    Type of Immunogen: Synthetic peptide - KLH conjugated

    Isotype

    IgG
  • Indications d'application

    Approved: IP, WB (0.5 - 2 μg/mL)

    Usage: Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Immunoprecipitation: 4 μg immunoprecipitated ADAP/SLAP-130/Fyb from 500 μg of RAW. 264.7 macrophage RIPA lysate.

    Not recommended for: IHC-P

    Commentaires

    Target Species of Antibody: Mouse

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, before the addition of glycerol to 30 %

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    avoid freeze thaw cycles.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • Antigène

    FYB (FYN-Binding Protein (FYB))

    Autre désignation

    ADAP / FYB

    Sujet

    Name/Gene ID: FYB

    Synonyms: FYB, ADAP, FYN-binding protein, FYN-T-binding protein, FYB-120/130, FYN binding protein, p120/p130, PRO0823, SLAP130, SLAP-130

    ID gène

    2533

    UniProt

    O15117

    Pathways

    TCR Signaling, Protein targeting to Nucleus
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